patrickPatrick Meraldi | Dept. Biochemistry, ETH-Zurich, Switzerland

Patrick is our main collaborator with regard to kinetochores, and has been for the past 6 years. This all began when we were both postdocs in Peter Sorger's lab at MIT. At the time I was well and truly a yeast biologist with little time for those larger eukaryotes. But, as time went on, I was slowly converted, mostly as a result of Patrick and I finding the human orthologues of yeast kinetochore proteins through sequence-search based approaches (Meraldi et al, Genome Biology, 2006). We both left Peter's lab in 2005 to establish our own labs with the aim of understanding the function of these new human kinetochore protein - a task that we decided to pursue as a collaborative effort. We have maintained this effort through joint projects, lots of great discussion and an annual joint lab retreat in the swiss mountains or in the english rain...you can find out more about Patrick's lab here

 

rob

Rob Cross | Centre for Mechanochemical Cell Biology

Our collaborations began when I established my lab at the Marie Curie Research Institute in 2005. We have a shared interest in understanding how molecular motors are able to drive self-organizaton of higher-order microtubule-based structures. Rob and I worked together to show that fission yeast kinesin-14 (Klp2) is able to sort microtubules into parallel bundles using an ATP-dependent mechanism (Braun et al, Nat. Cell Biol., 2009) and more recently about the choice of spindle assembly pathways in human cells (Kaseda et al, Biology Open, in press). We continue to advance this work by using a combination of protein engineering and knock-in technology to define how the biophysical properties of motor proteins drives cell biological processes

anne Anne Straube | Centre for Mechanochemical Cell Biology

Our labs have interacted closely since Anne began her lab at the Marie Curie Research Institute in 2007 as we have a common interest in live cel imaging and understanding microtubule-based processes. Over the last 3 years we have been investigating how the microtubule-associated proteins MAP4 and CLASP1 control astral-microtubule/cortex interactions (Samora et al, Nat. Cell Biol., 2011). We hope to gain new insight into the force-generating mechanisms that position in the spindle during mitosis thereby maintaining the correct cell division plane

 

rob Nigel Burroughs | Warwick Systems Biology Centre

Our collaborations with Nigel began following our move to Warwick in 2009. We are working towards building a new generation of mathematical model for chromosome dynamics in human cells. We have one shared PhD student (Ed Harry). This work is now funded by a new BBSRC grant which will allow us to expand this exciting area of work

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Selected Publications

Samora, C.P.*, Mogessie, B.*, Conway, L., Ross, J.L., Straube, A. and McAinsh, A.D. (2011) MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis.
Nature Cell Biology, Published online: 07 August 2011 | doi:10.1038/ncb2297

 

Amaro, A.C., Samora, C.P., Holtackers, R., Wang, E., Kingston, I., Alonso, M., Lampson, L., McAinsh, A.D. and Meraldi, P. (2010) Molecular control of kinetochore-microtubule dynamics and chromosome oscillation
Nature Cell Biology, 12: 319-329

 

*Jaqaman, K., *King, E., *Amaro, A.C., *Winter, J.R., Dorn, J.F., Elliott, H.L., Mchedlishvili, N., McClelland, S.E., Porter, I.M., Posch, M., Toso, A., Danuser, G., McAinsh, A.D., Meraldi, P. and Swedlow, J.R. (2010) Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases
Journal of Cell Biology, 188: 665-79

 

Braun, M., Drummond, D.R., Cross, R.A. and McAinsh, A.D. (2009)Klp2 organises microtubules into parallel bundles by an ATP-dependent sorting mechanism
Nature Cell Biology, 11: 724-730

*Toso, A., *Winter, J., Garrod, A.J., Amaro, A.C., Meraldi, P. and McAinsh, A.D. (2009) Kinetchore-generated pushing forces separate centrosomes during bipolar spindle assembly.
Journal Cell Biology, 184: 365-372

>> View my collection, "My Bibliography" from NCBI

 

>> what happened to past lab members? here

 

>> current collaborations NEW

 

>> image gallery under construction...

 

>> software under construction...

 

mitosisbookMitosis Book

May 2009 | Mitosis: Methods and Protocols is available for order. The book, edited by Andrew, includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. > more here

 


funding sources

 

mccc

mccc