kitKiT - kinetochore tracking software

KiT is a MATLAB program for tracking kinetochores in 2D or 3D, and in multiple channels (Armond et al, Bioinformatics (2016). KiT evolved from earlier software for particle tracking (u-Track; Jaqaman et al., 2008), kinetochore tracking (MaKi; Jaqaman et al., 2010) and kinetochore track analysis (CupL; Vladimirou et al., 2013) and incorporates numerous enhancements and major new features. Movies are loaded through the Bioformats package (Linkert et al., 2010), which is automatically downloaded, enabling compatibility with a vast range of microscopy data formats.

This software is licensed for use under the GNU GPL v3 license. See LICENSE file for details.

Download KiT 1.5.4 here.

17/10/16 - Updated version now available; includes several bug fixes

Download KiT 1.5.6 here.

Selected Papers


Drechsler, H., McHugh, T., Singleton, M.R., Carter, N.J. and McAinsh, A.D. (2014) The kinesin-12 Kif15 is a processive track-switching tetramer.
eLife, 3: e01724; PMID: 24668168


Vladimirou, E.*, Mchedlishvili, N.*, Gasic, I.*, Armond, J.W., Samora, C.P., Meraldi, P**. and McAinsh, A.D.** (2013) Nonautonomous movement of chromosomes in mitosis
Developmental Cell, 27: 60-71; PMID: 24135231


Samora, C.P.*, Mogessie, B.*, Conway, L., Ross, J.L., Straube, A.** and McAinsh, A.D.** (2011) MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis.
Nature Cell Biology, 13: 1040-50; PMID: 21822276


Amaro, A.C., Samora, C.P., Holtackers, R., Wang, E., Kingston, I., Alonso, M., Lampson, L., McAinsh, A.D.** and Meraldi, P.** (2010) Molecular control of kinetochore-microtubule dynamics and chromosome oscillation
Nature Cell Biology, 12: 319-329; PMID: 20228811


*Jaqaman, K., *King, E., *Amaro, A.C., *Winter, J.R., Dorn, J.F., Elliott, H.L., Mchedlishvili, N., McClelland, S.E., Porter, I.M., Posch, M., Toso, A., Danuser, G.**, McAinsh, A.D.**, Meraldi, P.** and Swedlow, J.R.** (2010) Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases
Journal of Cell Biology, 188: 665-79; PMID: 20212316


Braun, M., Drummond, D.R., Cross, R.A. and McAinsh, A.D. (2009) The kinesin-14 Klp2 organises microtubules into parallel bundles by an ATP-dependent sorting mechanism
Nature Cell Biology, 11: 724-730; PMID: 19430466

*Toso, A., *Winter, J., Garrod, A.J., Amaro, A.C., Meraldi, P.** and McAinsh, A.D.** (2009) Kinetchore-generated pushing forces separate centrosomes during bipolar spindle assembly.
Journal Cell Biology, 184: 365-372; PMID: 19204145

*equal contribution **corresponding authors